The Oncolytic Avian Reovirus p17 Protein Inhibits Invadopodia Formation in Murine Melanoma Cancer Cells by Suppressing the FAK/Src Pathway and the Formation of theTKs5/NCK1 Complex

• Published in: Viruses Vol. 16; no. 7; p. 1153
• Main Authors: Hsu, Chao-Yu, Li, Jyun-Yi, Yang, En-Ying, Liao, Tsai-Ling, Wen, Hsiao-Wei, Tsai, Pei-Chien, Ju, Tz-Chuen, Lye, Lon-Fye, Nielsen, Brent L., Liu, Hung-Jen
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.07.2024
• Subjects: Adaptor proteins; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Angiogenesis; Animals; Antibodies; Avian orthoreovirus; avian reoviruses; Breast cancer; Cancer; Cancer cells; Cell adhesion & migration; Cell cycle; Cell Line, Tumor; cell lines; Cell migration; Cell Movement; Cellular signal transduction; Efficiency; FAK/Src; Focal adhesion kinase; Focal Adhesion Kinase 1 - genetics; Focal Adhesion Kinase 1 - metabolism; Gelatinase B; Genomes; Health aspects; Kinases; Matrix metalloproteinase; Medical research; Medicine, Experimental; Melanoma; Melanoma, Experimental - pathology; Melanoma, Experimental - therapy; metalloproteinases; Metastasis; Mice; mutants; neoplasm cells; non-specific protein-tyrosine kinase; Oncolysis; Oncolytic Viruses - genetics; Oncolytic Viruses - physiology; Orthoreovirus, Avian - genetics; Orthoreovirus, Avian - physiology; p17; p17 Protein; p53 Protein; p53/PTEN; Phosphotransferases; Physiological aspects; Plasmids; Podosomes - metabolism; Proteins; PTEN Phosphohydrolase - genetics; PTEN Phosphohydrolase - metabolism; PTEN protein; Rab40b; Reoviruses; Signal Transduction; Src protein; src-Family Kinases - genetics; src-Family Kinases - metabolism; TKs5/NCK1 complex; transcription (genetics); Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; tyrosine; Viral proteins; Viral Proteins - genetics; Viral Proteins - metabolism; Wound healing; Taiwan; United States > US; Rab40b; invadopodia; FAK/Src; p17; TKs5/NCK1 complex; MMP9; avian reoviruses; p53/PTEN

To explore whether the p17 protein of oncolytic avian reovirus (ARV) mediates cell migration and invadopodia formation, we applied several molecular biological approaches for studying the involved cellular factors and signal pathways. We found that ARV p17 activates the p53/phosphatase and tensin ho...