Developmental validation of the AGCU 21+1 STR kit: A novel multiplex assay for forensic application

In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex‐determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D...

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Published in	Electrophoresis Vol. 36; no. 2; pp. 271 - 276
Main Authors	Zhu, Bo-Feng, Zhang, Yu-Dang, Shen, Chun-Mei, Du, Wei-An, Liu, Wen-Juan, Meng, Hao-Tian, Wang, Hong-Dan, Yang, Guang, Jin, Rui, Yang, Chun-Hua, Yan, Jiang-Wei, Bie, Xiao-Hua
Format	Journal Article
Language	English
Published	Germany Blackwell Publishing Ltd 01.01.2015
Subjects	AGCU 21+1 STR kit Animals Assaying Deoxyribonucleic acid DNA DNA genotyping DNA Primers electrophoresis Forensic engineering forensic sciences Forensic Sciences - methods Forensic validation Guidelines Humans Inhibitors Loci Magnesium Microsatellite Repeats Multiplex Polymerase Chain Reaction - methods Multiplexing polymerase chain reaction Reagent Kits, Diagnostic Sensitivity and Specificity Sex Determination Analysis Short tandem repeat Species Specificity Taq Polymerase Temperature AGCU 21+1 STR kit DNA genotyping Short tandem repeat Forensic validation
Online Access	Get full text
ISSN	0173-0835 1522-2683 1522-2683
DOI	10.1002/elps.201400333

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Abstract	In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex‐determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.
AbstractList	In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application. In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.
Author	Bie, Xiao-Hua Wang, Hong-Dan Yang, Chun-Hua Meng, Hao-Tian Yang, Guang Liu, Wen-Juan Jin, Rui Yan, Jiang-Wei Shen, Chun-Mei Zhu, Bo-Feng Du, Wei-An Zhang, Yu-Dang
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Snippet	In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a...
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SubjectTerms	AGCU 21+1 STR kit Animals Assaying Deoxyribonucleic acid DNA DNA genotyping DNA Primers electrophoresis Forensic engineering forensic sciences Forensic Sciences - methods Forensic validation Guidelines Humans Inhibitors Loci Magnesium Microsatellite Repeats Multiplex Polymerase Chain Reaction - methods Multiplexing polymerase chain reaction Reagent Kits, Diagnostic Sensitivity and Specificity Sex Determination Analysis Short tandem repeat Species Specificity Taq Polymerase Temperature
Title	Developmental validation of the AGCU 21+1 STR kit: A novel multiplex assay for forensic application
URI	https://api.istex.fr/ark:/67375/WNG-DSVF4TZ8-1/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1002%2Felps.201400333 https://www.ncbi.nlm.nih.gov/pubmed/25363755 https://www.proquest.com/docview/1652385427 https://www.proquest.com/docview/1660090729 https://www.proquest.com/docview/1733537777
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