Analysis of extracellular vesicle DNA at the single‐vesicle level by nano‐flow cytometry

• Published in: Journal of extracellular vesicles Vol. 11; no. 4; pp. e12206 - n/a
• Main Authors: Liu, Haisheng, Tian, Ye, Xue, Chengfeng, Niu, Qian, Chen, Chen, Yan, Xiaomei
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.04.2022; John Wiley and Sons Inc; Wiley
• Subjects: Cell culture; Cell size; Deoxyribonucleic acid; DNA; DNA - metabolism; DNase digestion; Exosomes; Extracellular vesicles; Extracellular Vesicles - metabolism; Flow Cytometry; Genotoxicity; Histones; microvesicles; Mitochondrial DNA; Nanoparticles; nano‐flow cytometry; Secretion; single particle analysis; Staining and Labeling; Ultracentrifugation; China; extracellular vesicles; DNA; exosomes; DNase digestion; microvesicles; single particle analysis; nano-flow cytometry

It has been demonstrated recently that extracellular vesicles (EVs) carry DNA; however, many fundamental features of DNA in EVs (EV‐DNA) remain elusive. In this study, a laboratory‐built nano‐flow cytometer (nFCM) that can detect single EVs as small as 40 nm in diameter and single DNA fragments of 2...