Establishment of a simplified dichotomic size‐exclusion chromatography for isolating extracellular vesicles toward clinical applications

• Published in: Journal of extracellular vesicles Vol. 10; no. 11; pp. e12145 - n/a
• Main Authors: Guo, Jiahui, Wu, Caihong, Lin, Xinyi, Zhou, Jian, Zhang, Jiayi, Zheng, Wenting, Wang, Tong, Cui, Yizhi
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.09.2021; John Wiley and Sons Inc; Wiley
• Subjects: Biomarkers; Chromatography; Chromatography, Gel - methods; clinical application; Contaminants; dichotomic size‐exclusion chromatography; Extracellular vesicles; Extracellular Vesicles - metabolism; human plasma/serum; Humans; Laboratories; Lipoproteins; Plasma; Proteins; Proteomics; Proteomics - methods; Resins; simplified; Ultracentrifugation; China; clinical application; human plasma/serum; dichotomic size-exclusion chromatography; extracellular vesicles; simplified

Size‐exclusion chromatography (SEC) is a widely adopted method for the isolation of extracellular vesicles (EVs) from complex samples. SEC can efficiently remove high‐abundant proteins, while often requires multiple fractionation operation using diversified column settings. In this study, we aim to...