Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy

• Published in: Cancer communications (London, England) Vol. 40; no. 4; pp. 135 - 153
• Main Authors: Tan, Wei Chang Colin, Nerurkar, Sanjna Nilesh, Cai, Hai Yun, Ng, Harry Ho Man, Wu, Duoduo, Wee, Yu Ting Felicia, Lim, Jeffrey Chun Tatt, Yeong, Joe, Lim, Tony Kiat Hon
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.04.2020; John Wiley and Sons Inc
• Subjects: Antibodies; Antigens; Apoptosis; Biomarkers; Cancer; Gene expression; immunofluorescence; immunohistochemistry; Immunotherapy; Ion beams; Ligands; Lymphocytes; Mass spectrometry; Microscopy; multiplex; overview; Product development; Review; Reviews; Scientific imaging; United States > US; Germany; overview; immunohistochemistry; multiplex; immunotherapy; immunofluorescence

Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per tissue section. This review details various h...