Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy

Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per tissue section. This review details various h...

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Published inCancer communications (London, England) Vol. 40; no. 4; pp. 135 - 153
Main Authors Tan, Wei Chang Colin, Nerurkar, Sanjna Nilesh, Cai, Hai Yun, Ng, Harry Ho Man, Wu, Duoduo, Wee, Yu Ting Felicia, Lim, Jeffrey Chun Tatt, Yeong, Joe, Lim, Tony Kiat Hon
Format Journal Article
LanguageEnglish
Published United States John Wiley & Sons, Inc 01.04.2020
John Wiley and Sons Inc
Subjects
Antibodies
Antigens
Apoptosis
Biomarkers
Cancer
Gene expression
immunofluorescence
immunohistochemistry
Immunotherapy
Ion beams
Ligands
Lymphocytes
Mass spectrometry
Microscopy
multiplex
overview
Product development
Review
Reviews
Scientific imaging
United States > US
Germany
overview
immunohistochemistry
multiplex
immunotherapy
immunofluorescence
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Summary:Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell‐cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high‐throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost‐effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.
Bibliography:These authors contributed equally to this work.
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ISSN:2523-3548
2523-3548
DOI:10.1002/cac2.12023