Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy

Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per tissue section. This review details various h...

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Published inCancer communications (London, England) Vol. 40; no. 4; pp. 135 - 153
Main Authors Tan, Wei Chang Colin, Nerurkar, Sanjna Nilesh, Cai, Hai Yun, Ng, Harry Ho Man, Wu, Duoduo, Wee, Yu Ting Felicia, Lim, Jeffrey Chun Tatt, Yeong, Joe, Lim, Tony Kiat Hon
Format Journal Article
LanguageEnglish
Published United States John Wiley & Sons, Inc 01.04.2020
John Wiley and Sons Inc
Subjects
Antibodies
Antigens
Apoptosis
Biomarkers
Cancer
Gene expression
immunofluorescence
immunohistochemistry
Immunotherapy
Ion beams
Ligands
Lymphocytes
Mass spectrometry
Microscopy
multiplex
overview
Product development
Review
Reviews
Scientific imaging
United States > US
Germany
overview
immunohistochemistry
multiplex
immunotherapy
immunofluorescence
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Abstract Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell‐cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high‐throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost‐effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.
AbstractList Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell‐cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high‐throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost‐effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.
Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter-observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell-cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost-effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter-observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell-cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost-effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.
Author Lim, Jeffrey Chun Tatt
Yeong, Joe
Wu, Duoduo
Wee, Yu Ting Felicia
Tan, Wei Chang Colin
Lim, Tony Kiat Hon
Ng, Harry Ho Man
Nerurkar, Sanjna Nilesh
Cai, Hai Yun
AuthorAffiliation 2 Department of Anatomical Pathology Singapore General Hospital Singapore 169856 Singapore
3 Institute of Molecular Cell Biology (IMCB), Agency of Science, Technology and Research (ASTAR) Singapore 169856 Singapore
5 Duke‐NUS Medical School Singapore 169856 Singapore
1 Yong Loo Lin School of Medicine National University of Singapore Singapore 169856 Singapore
4 Singapore Immunology Network Agency of Science (SIgN) Technology and Research (ASTAR) Singapore 169856 Singapore
AuthorAffiliation_xml – name: 2 Department of Anatomical Pathology Singapore General Hospital Singapore 169856 Singapore
– name: 5 Duke‐NUS Medical School Singapore 169856 Singapore
– name: 1 Yong Loo Lin School of Medicine National University of Singapore Singapore 169856 Singapore
– name: 4 Singapore Immunology Network Agency of Science (SIgN) Technology and Research (ASTAR) Singapore 169856 Singapore
– name: 3 Institute of Molecular Cell Biology (IMCB), Agency of Science, Technology and Research (ASTAR) Singapore 169856 Singapore
Author_xml – sequence: 1
  givenname: Wei Chang Colin
  surname: Tan
  fullname: Tan, Wei Chang Colin
  organization: National University of Singapore
– sequence: 2
  givenname: Sanjna Nilesh
  surname: Nerurkar
  fullname: Nerurkar, Sanjna Nilesh
  organization: National University of Singapore
– sequence: 3
  givenname: Hai Yun
  surname: Cai
  fullname: Cai, Hai Yun
  organization: National University of Singapore
– sequence: 4
  givenname: Harry Ho Man
  surname: Ng
  fullname: Ng, Harry Ho Man
  organization: Duke‐NUS Medical School
– sequence: 5
  givenname: Duoduo
  surname: Wu
  fullname: Wu, Duoduo
  organization: National University of Singapore
– sequence: 6
  givenname: Yu Ting Felicia
  surname: Wee
  fullname: Wee, Yu Ting Felicia
  organization: Singapore General Hospital
– sequence: 7
  givenname: Jeffrey Chun Tatt
  surname: Lim
  fullname: Lim, Jeffrey Chun Tatt
  organization: Institute of Molecular Cell Biology (IMCB), Agency of Science, Technology and Research (ASTAR)
– sequence: 8
  givenname: Joe
  surname: Yeong
  fullname: Yeong, Joe
  email: yeongps@imcb.a-star.edu.sg
  organization: Technology and Research (ASTAR)
– sequence: 9
  givenname: Tony Kiat Hon
  surname: Lim
  fullname: Lim, Tony Kiat Hon
  email: lim.kiat.hon@singhealth.com.sg
  organization: Singapore General Hospital
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32301585$$D View this record in MEDLINE/PubMed
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Copyright 2020 The Authors. published by John Wiley & Sons Australia, Ltd. on behalf of Sun Yat‐sen University Cancer Center
2020 The Authors. Cancer Communications published by John Wiley & Sons Australia, Ltd. on behalf of Sun Yat-sen University Cancer Center.
2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: 2020 The Authors. published by John Wiley & Sons Australia, Ltd. on behalf of Sun Yat‐sen University Cancer Center
– notice: 2020 The Authors. Cancer Communications published by John Wiley & Sons Australia, Ltd. on behalf of Sun Yat-sen University Cancer Center.
– notice: 2020. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
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Issue 4
Keywords overview
immunohistochemistry
multiplex
immunotherapy
immunofluorescence
Language English
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2020 The Authors. Cancer Communications published by John Wiley & Sons Australia, Ltd. on behalf of Sun Yat-sen University Cancer Center.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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Snippet Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of...
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SubjectTerms Antibodies
Antigens
Apoptosis
Biomarkers
Cancer
Gene expression
immunofluorescence
immunohistochemistry
Immunotherapy
Ion beams
Ligands
Lymphocytes
Mass spectrometry
Microscopy
multiplex
overview
Product development
Review
Reviews
Scientific imaging
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  priority: 102
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Title Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fcac2.12023
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Volume 40
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