Selective Transcription of an Unnatural Naphthyridine:Imidazopyridopyrimidine Base Pair Containing Four Hydrogen Bonds with T7 RNA Polymerase

• Published in: Angewandte Chemie International Edition Vol. 53; no. 47; pp. 12844 - 12848
• Main Authors: Nomura, Yusaku, Kashiwagi, Satoshi, Sato, Kousuke, Matsuda, Akira
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 17.11.2014; WILEY‐VCH Verlag; Wiley; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Base Pairing; Biocatalysis; Chemistry; Chemistry, Multidisciplinary; Deoxyribonucleic acid; DNA polymerase; DNA-Directed RNA Polymerases - metabolism; Genes; Human; Humans; Hydrogen Bonding; Hydrogen bonds; Molecular Structure; Naphthyridines - chemistry; Naphthyridines - metabolism; nucleobases; nucleotides; Physical Sciences; Polymerase; Pyrimidines - chemistry; Pyrimidines - metabolism; Ribonucleic acids; RNA polymerase; RNA recognition; Science & Technology; Synthesis; unnatural base pairs; Viral Proteins - metabolism; OLIGODEOXYNUCLEOTIDES; NUCLEOTIDE INCORPORATION; RNA recognition; CRYSTAL-STRUCTURES; DNA-POLYMERASE; hydrogen bonds; REPLICATION; nucleobases; STRUCTURAL BASIS; HYDROPHOBIC BASE; GENETIC ALPHABET; ENZYMATIC INCORPORATION; SITE-SPECIFIC INCORPORATION; nucleotides; unnatural base pairs

The naphthyridine:imidazopyridopyrimidine base pair is the first base pair containing four hydrogen bonds that can be replicated selectively and efficiently by the use of DNA polymerases. Herein we describe the synthesis of naphthyridine‐C‐ribonucleoside 5′‐triphosphate (rNaTP) and transcription rea...