Spatially coordinated kinase signaling regulates local axon degeneration
In addition to being a hallmark of neurodegenerative disease, axon degeneration is used during development of the nervous system to prune unwanted connections. In development, axon degeneration is tightly regulated both temporally and spatially. Here, we provide evidence that degeneration cues are t...
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Published in | The Journal of neuroscience Vol. 32; no. 39; pp. 13439 - 13453 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Society for Neuroscience
26.09.2012
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Subjects | |
Online Access | Get full text |
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Summary: | In addition to being a hallmark of neurodegenerative disease, axon degeneration is used during development of the nervous system to prune unwanted connections. In development, axon degeneration is tightly regulated both temporally and spatially. Here, we provide evidence that degeneration cues are transduced through various kinase pathways functioning in spatially distinct compartments to regulate axon degeneration. Intriguingly, glycogen synthase kinase-3 (GSK3) acts centrally, likely modulating gene expression in the cell body to regulate distally restricted axon degeneration. Through a combination of genetic and pharmacological manipulations, including the generation of an analog-sensitive kinase allele mutant mouse for GSK3β, we show that the β isoform of GSK3, not the α isoform, is essential for developmental axon pruning in vitro and in vivo. Additionally, we identify the dleu2/mir15a/16-1 cluster, previously characterized as a regulator of B-cell proliferation, and the transcription factor tbx6, as likely downstream effectors of GSK3β in axon degeneration. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: M.C., J.W.L., R.M.W., and R.J.W. designed research; M.C., J.A.M., D.K., J.K.A., S.J.T., and R.M.W. performed research; K.B. and H.K. contributed unpublished reagents/analytic tools; M.C., J.S.K., and R.J.W. analyzed data; M.C. and R.J.W. wrote the paper. |
ISSN: | 0270-6474 1529-2401 1529-2401 |
DOI: | 10.1523/jneurosci.2039-12.2012 |