MAPK Phosphatase-2 (MKP-2) Is Induced by hCG and Plays a Role in the Regulation of CYP11A1 Expression in MA-10 Leydig Cells

• Published in: Endocrinology (Philadelphia) Vol. 154; no. 4; pp. 1488 - 1500
• Main Authors: Gómez, Natalia V, Gorostizaga, Alejandra B, Mori Sequeiros García, María M, Brion, Laura, Acquier, Andrea, González-Calvar, Silvia I, Méndez, Carlos F, Podestá, Ernesto J, Paz, Cristina
• Format: Journal Article
• Language: English
• Published: Chevy Chase, MD Endocrine Society 01.04.2013; Oxford University Press
• Subjects: 8-Bromo Cyclic Adenosine Monophosphate - metabolism; Accumulation; Animals; Biological and medical sciences; Cell activation; Cell Line, Tumor; Chimeras; Cholesterol Side-Chain Cleavage Enzyme - metabolism; Chorionic gonadotropin; Chorionic Gonadotropin - metabolism; Cyclic AMP; Down-regulation; Extracellular signal-regulated kinase; Flags; Fundamental and applied biological sciences. Psychology; Gene regulation; Gonadotropins; Kinases; Leydig cells; Leydig Cells - enzymology; Male; MAP kinase; Medicin och hälsovetenskap; Mice; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - metabolism; MKP-2 protein; mRNA; Phosphatase; Phosphorylation; Pituitary (anterior); Protein folding; Protein kinase A; Protein Tyrosine Phosphatases - metabolism; Proteins; Real-Time Polymerase Chain Reaction; Receptor mechanisms; Receptors, LH - metabolism; RNA, Messenger - analysis; RNA, Messenger - metabolism; Stimulation; Up-Regulation; Vertebrates: endocrinology; Human chorionic gonadotrophin; Enzyme; Transferases; Mitogen-activated protein kinase; Gonadotropin; Testicle; Male genital system; Placental hormone; Leydig cell

MAPKs such as ERK1/2 are dephosphorylated, and consequently inactivated, by dual specificity phosphatases (MKPs). In Leydig cells, LH triggers ERK1/2 phosphorylation through the action of protein kinase A. We demonstrate that, in MA-10 Leydig cells, LH receptor activation by human chorionic gonadotr...