Rapid extraction of secretin from plasma by XAD-2 resin and its application in the radioimmunoassay of secretin
A simple and rapid method for extraction of plasma secretin has been developed. In this method plasma was allowed to percolate through a XAD-2 resin (a copolymer of styrene and divinylbenzene) column, and secretin was eluted by acid methanol in good yield. The column extract showed little interferen...
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| Published in | Analytical biochemistry Vol. 87; no. 2; pp. 376 - 385 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
01.07.1978
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0003-2697 1096-0309 |
| DOI | 10.1016/0003-2697(78)90687-5 |
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| Abstract | A simple and rapid method for extraction of plasma secretin has been developed. In this method plasma was allowed to percolate through a XAD-2 resin (a copolymer of styrene and divinylbenzene) column, and secretin was eluted by acid methanol in good yield. The column extract showed little interference in the radioimmunoassay as evidenced by the superimposability of the standard curve prepared in the presence of the column extract to that prepared in the buffer alone. Furthermore, the bound-to-total ratios of radioactive secretin were not affected by the presence of column extracts of secretin-free plasma from different subjects. Using the method described, we were able to detect significant increases in plasma secretin levels in both dogs and humans following intraduodenal infusion of 0.05
n hydrochloric acid in much smaller quantities than those reported in the literature. |
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| AbstractList | A simple and rapid method for extraction of plasma secretin has been developed. In this method plasma was allowed to percolate through a XAD-2 resin (a copolymer of styrene and divinylbenzene) column, and secretin was eluted by acid methanol in good yield. The column extract showed little interference in the radioimmunoassay as evidenced by the superimposability of the standard curve prepared in the presence of the column extract to that prepared in the buffer alone. Furthermore, the bound-to-total ratios of radioactive secretin were not affected by the presence of column extracts of secretin-free plasma from different subjects. Using the method described, we were able to detect significant increases in plasma secretin levels in both dogs and humans following intraduodenal infusion of 0.05
n hydrochloric acid in much smaller quantities than those reported in the literature. |
| Author | Chey, William Y. Tai, Hsin-Hsiung |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/686358$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1210/endo-92-6-1617 10.1016/0006-291X(75)90151-5 10.1016/S0021-9258(19)41847-4 10.1016/S0021-9673(01)85641-7 10.1002/cber.19721050811 10.1016/0003-2697(76)90305-5 10.1016/0005-2795(73)90190-6 10.3891/acta.chem.scand.15-1790 10.1016/S0039-128X(68)80139-4 10.1136/gut.15.9.690 10.1016/S0016-5085(77)80303-X 10.1016/0003-2697(75)90562-X 10.1210/jcem-33-2-254 10.3109/00365517609055266 10.1016/S0021-9258(19)45802-X 10.1007/BF01072080 10.3891/acta.chem.scand.13-1247 10.1152/ajplegacy.1976.230.3.784 |
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| SubjectTerms | Animals Cattle Chromatography, Ion Exchange - methods Duodenum - physiology Fasting Humans Hydrochloric Acid - pharmacology Radioimmunoassay - methods Secretin - blood Secretin - isolation & purification Swine |
| Title | Rapid extraction of secretin from plasma by XAD-2 resin and its application in the radioimmunoassay of secretin |
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