A Heterotrimeric G Protein Complex Couples the Muscarinic m1 Receptor to Phospholipase C-β

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 93; no. 4; pp. 1391 - 1396
• Main Authors: Dippel, Edgar, Kalkbrenner, Frank, Wittig, Burghardt, Schultz, Günter
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 20.02.1996; National Acad Sciences; National Academy of Sciences
• Subjects: Animals; Antisense oligonucleotides; Base Sequence; Carbachol - pharmacology; Cell lines; Cells; Cellular biology; CHO cells; COS cells; DNA, Complementary - genetics; Enzyme Activation; GTP-Binding Proteins - metabolism; HEK293 cells; Isoenzymes - metabolism; Leukemia; Leukemia, Basophilic, Acute - pathology; Macromolecular Substances; Messenger RNA; Molecular Sequence Data; Muscarinic Antagonists - pharmacology; Oligonucleotides; Oligonucleotides, Antisense - pharmacology; Phospholipase C beta; Protein subunits; Proteins; Rats; Receptors; Receptors, Muscarinic - metabolism; Recombinant Proteins - metabolism; Rodents; Transfection; Tumor Cells, Cultured; Type C Phospholipases - metabolism

We addressed the question as to which subtypes of G protein subunits mediate the activation of phospholipase C-β by the muscarinic m1 receptor. We used the rat basophilic leukemia cell line RBL-2H3-hm1 stably transfected with the human muscarinic m1 receptor cDNA. We microinjected antisense oligonuc...