The Minimum Significant Ratio: A Statistical Parameter to Characterize the Reproducibility of Potency Estimates from Concentration-Response Assays and Estimation by Replicate-Experiment Studies

• Published in: Journal of biomolecular screening Vol. 11; no. 3; pp. 253 - 261
• Main Authors: Eastwood, Brian J., Farmen, Mark W., Iversen, Philip W., Craft, Trelia J., Smallwood, Jeffrey K., Garbison, Kim E., Delapp, Neil W., Smith, Gerald F.
• Format: Journal Article
• Language: English
• Published: United States 01.04.2006
• Subjects: Factor Xa - metabolism; Factor Xa Inhibitors; Models, Statistical; Reproducibility of Results

The authors show by illustration that procedures used to validate the reliability of single-concentration high-throughput screens such as the signal window and Z' factor do not ensure sufficient reliability in potency estimates from concentration response assays. They develop the minimum signif...