Accelerated High-Yield Generation of Limb-Innervating Motor Neurons from Human Stem Cells

• Published in: The Journal of neuroscience Vol. 33; no. 2; pp. 574 - 586
• Main Authors: Amoroso, Mackenzie W., Croft, Gist F., Williams, Damian J., O'Keeffe, Sean, Carrasco, Monica A., Davis, Anne R., Roybon, Laurent, Oakley, Derek H., Maniatis, Tom, Henderson, Christopher E., Wichterle, Hynek
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 09.01.2013
• Subjects: Animals; Axons - physiology; Calcium - physiology; Calcium Signaling - physiology; Cell Differentiation - physiology; Cells, Cultured; Chick Embryo; DNA, Complementary - biosynthesis; DNA, Complementary - genetics; Extremities - innervation; Female; Forkhead Transcription Factors - biosynthesis; Forkhead Transcription Factors - genetics; Homeodomain Proteins - genetics; Humans; Immunohistochemistry; LIM-Homeodomain Proteins - genetics; Male; Mice; Motor Neurons - metabolism; Motor Neurons - physiology; Neural Stem Cells - metabolism; Neural Stem Cells - physiology; Patch-Clamp Techniques; Repressor Proteins - biosynthesis; Repressor Proteins - genetics; RNA-Induced Silencing Complex; Spinal Cord - cytology; Spinal Cord - embryology; Stem Cell Transplantation - methods; Transcription Factors - genetics

Human pluripotent stem cells are a promising source of differentiated cells for developmental studies, cell transplantation, disease modeling, and drug testing. However, their widespread use even for intensely studied cell types like spinal motor neurons is hindered by the long duration and low yiel...