Isolation of phosphopeptides using zirconium-chlorophosphonazo chelate-modified silica nanoparticles

• Published in: Journal of Chromatography A Vol. 1218; no. 18; pp. 2528 - 2539
• Main Authors: Zhao, Pei-Xuan, Zhao, Yong, Guo, Xiao-Feng, Wang, Hong, Zhang, Hua-Shan
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06.05.2011; Elsevier
• Subjects: Affinity; alpha-casein; Animals; beta-casein; Biological and medical sciences; biomarkers; blood serum; bovine serum albumin; Cattle; Chelating; Chelating Agents - chemistry; chromatography; Chromatography, Affinity; desorption; detection limit; diabetes; disease diagnosis; Enrichment; Human serum; Humans; Hydrogen-Ion Concentration; hypertension; Investigative techniques, diagnostic techniques (general aspects); ionization; ions; MALDI-TOF MS; Materials selection; matrix-assisted laser desorption-ionization mass spectrometry; Medical sciences; metal ions; Miscellaneous. Technology; Nanoparticles; Nanoparticles - chemistry; Naphthalenesulfonates - chemistry; ovalbumin; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; people; Peptide Fragments - isolation & purification; Phosphopeptide enrichment; Phosphopeptides - isolation & purification; phosphoproteins; Phosphorylation; Protein Binding; protein phosphorylation; Proteins - isolation & purification; screening; silica; Silicon Dioxide; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Zirconium; Zirconium - chemistry; Zirconium-chlorophosphonazo chelate-modified silica nanoparticles; Human serum; MALDI-TOF MS; Zirconium-chlorophosphonazo chelate-modified silica nanoparticles; Phosphopeptide enrichment; Biological fluid; Phenolate; Nanoparticle; Biological marker; Matrix assisted laser desorption ionization; Silica; Blood; Chemical enrichment; Azo compound; Clinical biology; Qualitative analysis; Diagnosis; Adsorbent; Modified material; Human; Hypertension; Purification; Healthy subject; Diabetes mellitus; Phosphopeptide; Organic ligand; Patient; Zirconium Complexes; Organic phosphonate; Transition metal Complexes; Aqua complex; Time of flight method; Preparation; Isolation; Blood serum; Mass spectrometry

Due to the low abundance of phosphoproteins and substoichiometry of phosphorylation, the elucidation of protein phosphorylation requires highly specific materials for isolation of phosphopeptides from biological samples prior to mass spectrometric analysis. In this study, chlorophosphonazo type deri...