A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins

• Published in: Communications biology Vol. 4; no. 1; pp. 777 - 11
• Main Authors: Liu, Si-Si, Liu, Yi-Shi, Guo, Xin-Yu, Murakami, Yoshiko, Yang, Ganglong, Gao, Xiao-Dong, Kinoshita, Taroh, Fujita, Morihisa
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.06.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/31; 42/40; 45/41; 631/45/221; 631/80/221; Biology; Biomedical and Life Sciences; Biosynthesis; Cell surface; Glycosylphosphatidylinositol; Life Sciences; Lipid rafts; Mammalian cells; Phosphatidylinositol; Phospholipase C; Proteins

Over 100 kinds of proteins are expressed as glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) on the cell surface in mammalian cells. GPI-APs possess unique properties in terms of their intracellular trafficking and association with lipid rafts. Although it is clear that GPI-APs play cr...