Comparative analysis of SARS-CoV-2 neutralization titers reveals consistency between human and animal model serum and across assays
The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires ongoing monitoring to judge the ability of newly arising variants to escape the immune response. A surveillance system necessitates an understanding of differences in neutralization titers measured in different as...
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| Published in | Science translational medicine Vol. 16; no. 747; p. eadl1722 |
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| Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
15.05.2024
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| Subjects | |
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| Abstract | The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires ongoing monitoring to judge the ability of newly arising variants to escape the immune response. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal serum samples. We compared 18 datasets generated using human, hamster, and mouse serum and six different neutralization assays. Datasets using animal model serum samples showed higher titer magnitudes than datasets using human serum samples in this comparison. Fold change in neutralization of variants compared to ancestral SARS-CoV-2, immunodominance patterns, and antigenic maps were similar among serum samples and assays. Most assays yielded consistent results, except for differences in fold change in cytopathic effect assays. Hamster serum samples were a consistent surrogate for human first-infection serum samples. These results inform the transition of surveillance of SARS-CoV-2 antigenic variation from dependence on human first-infection serum samples to the utilization of serum samples from animal models. |
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| AbstractList | The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires ongoing monitoring to judge the ability of newly arising variants to escape the immune response. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal serum samples. We compared 18 datasets generated using human, hamster, and mouse serum and six different neutralization assays. Datasets using animal model serum samples showed higher titer magnitudes than datasets using human serum samples in this comparison. Fold change in neutralization of variants compared to ancestral SARS-CoV-2, immunodominance patterns, and antigenic maps were similar among serum samples and assays. Most assays yielded consistent results, except for differences in fold change in cytopathic effect assays. Hamster serum samples were a consistent surrogate for human first-infection serum samples. These results inform the transition of surveillance of SARS-CoV-2 antigenic variation from dependence on human first-infection serum samples to the utilization of serum samples from animal models. |
| Author | Rössler, Annika Douek, Daniel C Mykytyn, Anna Shen, Xiaoying Frieman, Matthew Godbole, Sucheta Krammer, Florian Jones, Terry C Davis-Gardner, Meredith E Wang, Wei Corman, Victor M Supasa, Piyada Lai, Lilin Carreño, Juan Manuel Katzelnick, Leah C Kimpel, Janine Screaton, Gavin R Baracco, Lauren Lusvarghi, Sabrina Weiss, Carol D Eckerle, Isabella Ellis, Madison Simon, Viviana Netzl, Antonia Haagmans, Bart Liu, Chang Drosten, Christian Suthar, Mehul S Edara, Venkata-Viswanadh Henry, Amy R Wilks, Samuel H Dejnirattisai, Wanwisa Smith, Derek J Mühlemann, Barbara Fouchier, Ron A M Kawaoka, Yoshihiro Halfmann, Peter J Meyer, Benjamin Sigal, Alex Montefiori, David C Bekliz, Meriem Diamond, Michael S Pollett, Simon Subramanian, Rahul Türeli, Sina Mongkolsapaya, Juthathip |
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| SubjectTerms | Animals Antibodies, Neutralizing - blood Antibodies, Neutralizing - immunology Antibodies, Viral - blood Antibodies, Viral - immunology COVID-19 - blood COVID-19 - immunology COVID-19 - virology Cricetinae Disease Models, Animal Humans Mice Neutralization Tests SARS-CoV-2 - immunology |
| Title | Comparative analysis of SARS-CoV-2 neutralization titers reveals consistency between human and animal model serum and across assays |
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