T-cell, antibody, and cytokine responses to homologs of the 60-kilodalton heat shock protein in Helicobacter pylori infection

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Published inClinical and Diagnostic Laboratory Immunology Vol. 4; no. 4; pp. 440 - 446
Main Authors Sharma, S A, Miller, G G, Peek, Jr, R A, Pérez-Pérez, G, Blaser, M J
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.07.1997
Subjects
Adult
AIDS/HIV
Antibodies, Bacterial - blood
Biomarkers
CD4-Positive T-Lymphocytes - chemistry
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - microbiology
CD8-Positive T-Lymphocytes - chemistry
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - microbiology
Chaperonin 60 - immunology
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Helicobacter Infections - immunology
Helicobacter pylori - immunology
HLA-DR Antigens - immunology
Humans
Immunization
Immunoblotting
Immunophenotyping
Interferon-gamma - immunology
Interleukin-10 - immunology
Receptors, Interleukin-2 - immunology
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Abstract Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue CVI About CVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy CVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 1556-6811 Online ISSN: 1556-679X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to CVI .asm.org, visit: CVI       
AbstractList For Helicobacter pylori, the hsp60 heat shock protein encoded by hspB is being considered as a potential candidate for subunit vaccines. We investigated the humoral and cellular responses to H. pylori hsp60 and its cross-reactivity with the homologous Mycobacterium bovis p65 protein and autologous human hsp60 protein. H. pylori-infected persons had significantly higher levels than uninfected persons of serum immunoglobulin G antibodies recognizing H. pylori hsp60, but not M. bovis p65 or human hsp60, as determined by enzyme-linked immunosorbent assay. In contrast, immunoblotting demonstrated cross-reactivity of H. pylori hsp60 with human hsp60. T-cell recognition of H. pylori hsp60 was found in both infected and uninfected subjects, and there was no recognition of human hsp60. T cells from infected and uninfected subjects that had been activated in response to H. pylori hsp60 or M. bovis p65 were phenotypically similar but appeared to secrete different levels of gamma interferon and interleukin-10. These results demonstrate an apparent difference in the epitopes recognized by the T and B cells responding to H. pylori hsp60 in H. pylori-infected persons. In contrast to the T-cell responses, which were highly variable in all subjects and showed no recognition of autologous proteins, a specific B-cell response that may have cross-reactivity to human hsp60 is evident in some infected subjects.
Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue CVI About CVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy CVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 1556-6811 Online ISSN: 1556-679X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to CVI .asm.org, visit: CVI       
Author M J Blaser
R A Peek Jr
S A Sharma
G G Miller
G Pérez-Pérez
AuthorAffiliation Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
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Snippet Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon...
For Helicobacter pylori, the hsp60 heat shock protein encoded by hspB is being considered as a potential candidate for subunit vaccines. We investigated the...
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StartPage 440
SubjectTerms Adult
AIDS/HIV
Antibodies, Bacterial - blood
Biomarkers
CD4-Positive T-Lymphocytes - chemistry
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - microbiology
CD8-Positive T-Lymphocytes - chemistry
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - microbiology
Chaperonin 60 - immunology
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Helicobacter Infections - immunology
Helicobacter pylori - immunology
HLA-DR Antigens - immunology
Humans
Immunization
Immunoblotting
Immunophenotyping
Interferon-gamma - immunology
Interleukin-10 - immunology
Receptors, Interleukin-2 - immunology
Title T-cell, antibody, and cytokine responses to homologs of the 60-kilodalton heat shock protein in Helicobacter pylori infection
URI http://cvi.asm.org/content/4/4/440.abstract
https://www.ncbi.nlm.nih.gov/pubmed/9220161
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https://pubmed.ncbi.nlm.nih.gov/PMC170547
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