Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair

• Published in: The Journal of biological chemistry Vol. 295; no. 34; pp. 12214 - 12223
• Main Authors: Raducanu, Vlad-Stefan, Isaioglou, Ioannis, Raducanu, Daniela-Violeta, Merzaban, Jasmeen S., Hamdan, Samir M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.08.2020; American Society for Biochemistry and Molecular Biology
• Subjects: blot membrane; Denaturing Gradient Gel Electrophoresis; fluorescence; Fluorescent Dyes - chemistry; His-tag detection; histidine; Histidine - analysis; Histidine - chemistry; Humans; imaging; metal-ion-chelating nitrilotriacetate (NTA); Methods and Resources; PAGE; physical method; Recombinant Fusion Proteins - analysis; Recombinant Fusion Proteins - chemistry; recombinant protein expression; Small Ubiquitin-Related Modifier Proteins - analysis; Small Ubiquitin-Related Modifier Proteins - chemistry; Small Ubiquitin-Related Modifier Proteins - genetics; Ultraviolet Rays; UV detection of His-tag protein; UV transilluminator; UVHis-PAGE; Western blot; Western blot; UV detection of His-tag protein; physical method; recombinant protein expression; PAGE; blot membrane; imaging; UV transilluminator; His-tag detection; UVHis-PAGE; fluorescence; metal-ion-chelating nitrilotriacetate (NTA); histidine

The polyhistidine tag (His-tag) is one of the most popular protein tags used in the life sciences. Traditionally, the detection of His-tagged proteins relies on immunoblotting with anti-His antibodies. This approach is laborious for certain applications, such as protein purification, where time and...