Dysregulation of the miRNA biogenesis components DICER1, DROSHA, DGCR8 and AGO2 in clear cell renal cell carcinoma in both a Korean cohort and the cancer genome atlas kidney clear cell carcinoma cohort

• Published in: Oncology letters Vol. 18; no. 4; pp. 4337 - 4345
• Main Authors: Lee, Sang Su, Min, Hyeonji, Ha, Ji Yong, Kim, Byung Hoon, Choi, Mi Sun, Kim, Shin
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.10.2019; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Biosynthesis; Cancer; Cancer genetics; Carcinoma; Cluster analysis; Colorectal cancer; Datasets; Gene expression; Genes; Genetic aspects; Genomes; Genomics; Kidney cancer; Messenger RNA; Metastasis; MicroRNA; MicroRNAs; Oncology; Professional associations; Renal cell carcinoma; RNA; Statistical analysis; Surgery; South Korea; United States > US; the cancer genome atlas; clear cell renal cell carcinoma; miRNA biogenesis
• DOI: 10.3892/ol.2019.10759
• ISSN: 1792-1074

Impairment of microRNA (miRNA) biogenesis may be involved in clear cell renal cell carcinoma (ccRCC). The objective of the present study was to investigate the mRNA levels of important miRNA machinery components, , DiGeroge syndrome critical region gene 8 ( ), and Argonaute 2 ( ), and their correlations with clinicopathological characteristics of ccRCC using mRNA expression data from The Cancer Genome Atlas kidney clear cell carcinoma (TCGA KIRC) cohort and a Korean ccRCC cohort. mRNA levels of , and were significantly decreased in both cohorts. However, was significantly downregulated only in the Korean ccRCC cohort. Additionally, positive correlations were observed between the altered mRNA levels of and as well as and in both cohorts. In the TCGA KIRC cohort, alterations in the mRNA levels of were significantly correlated with histological grade. Furthermore, the altered mRNA levels of showed significant associations with sex and histologic grades. However, in the Korean ccRCC cohort, no factors were significantly associated with any clinicopathological parameters, including sex, age, T stage, Fuhrman grade/The International Society of Urological Pathology grade, lymphovascular invasion, and peri-renal fat invasion. Taken together, these findings indicate that and are significantly dysregulated in ccRCC, suggesting that they are important in the pathophysiology of this malignancy.