A Novel Method for Rapid Screening of Salmonidae Ingredients and Accurate Detection of Atlantic Salmon (Salmo salar) Simultaneously Using Duplex Real-Time PCR Coupled with Melting Curve Analysis

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 20; p. 4904
• Main Authors: Wang, Shihui, Xiong, Xiong, Song, Hongwei, Wang, Tianlong, Li, Yi, Wang, Libin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.10.2024; MDPI
• Subjects: Animals; Clothing industry; DNA Primers; duplex PCR; Fish; Health aspects; Identification; melting curve analysis; Methods; Protozoa; real-time PCR; Real-Time Polymerase Chain Reaction - methods; Salmo salar; Salmo salar - genetics; Salmon; Salmonidae; Salmonidae - genetics; Sensitivity and Specificity; Transition Temperature; Italy; China; Salmonidae; duplex PCR; melting curve analysis; real-time PCR; Salmo salar

The substitution of ingredients with Salmonidae, particularly Salmo salar, has led to widespread reports of financial losses and health risks globally, emphasizing the urgent need for the development of a rapid and precise method for species identification. The aim of the present study was to develo...