A Primitive Pathway of Porphyrin Biosynthesis and Enzymology in Desulfovibrio vulgaris

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 95; no. 9; pp. 4853 - 4858
• Main Authors: Ishida, Tetsuo, Yu, Ling, Akutsu, Hideo, Ozawa, Kiyoshi, Kawanishi, Shosuke, Seto, Akira, Inubushi, Toshiro, Sano, Seiyo
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 28.04.1998; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences
• Subjects: Absorption spectra; Acetates; Bacteria; Biochemistry; Biological Sciences; Biosynthesis; Chromatography, Thin Layer; Coproporphyrinogens; Coproporphyrinogens - metabolism; Coproporphyrins; Culture Media; Desulfovibrio vulgaris - metabolism; Enzymes; Esters; Lactones - chemistry; Mass Spectrometry; Molecular Weight; Porphyrins; Porphyrins - biosynthesis; Porphyrins - chemistry; Protoporphyrins; Spectrophotometry; Uroporphyrinogens; Uroporphyrinogens - metabolism; Uroporphyrins

Culture of Desulfovibrio vulgaris in a medium supplemented with 5-aminolevulinic acid and L-methionine-methyl-d3resulted in the formation of porphyrins (sirohydrochlorin, coproporphyrin III, and protoporphyrin IX) in which the methyl groups at the C-2 and C-7 positions were deuterated. A previously...