Gene expression profile of cytokines and receptors of inflammation from cultured keratinocytes of burned patients

• Published in: Burns Vol. 40; no. 5; pp. 947 - 956
• Main Authors: Gragnani, Alfredo, Cezillo, Marcus V.B, da Silva, Ismael D.C.G, de Noronha, Samuel M. Ribeiro, Correa-Noronha, Silvana A.A, Ferreira, Lydia M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.08.2014
• Subjects: Adult; Burns; Burns - genetics; Burns - immunology; Case-Control Studies; Cells, Cultured; Chemokines, C - genetics; Chemokines, C - immunology; Chemokines, CC - genetics; Chemokines, CC - immunology; Chemokines, CXC - genetics; Chemokines, CXC - immunology; Critical Care; Cytokines; Cytokines - genetics; Cytokines - immunology; Down-Regulation; Female; Gene expression; Gene Expression Profiling; Humans; Inflammation; Inflammation - genetics; Inflammation - immunology; Inflammation Mediators - immunology; Inflammation Mediators - metabolism; Interleukin-13 - genetics; Interleukin-13 - immunology; Interleukin-13 Receptor alpha1 Subunit - genetics; Interleukin-13 Receptor alpha1 Subunit - immunology; Interleukin-1beta - genetics; Interleukin-1beta - immunology; Interleukin-5 Receptor alpha Subunit - genetics; Interleukin-5 Receptor alpha Subunit - immunology; Interleukin-8 - genetics; Interleukin-8 - immunology; Keratinocytes; Keratinocytes - immunology; Keratinocytes - metabolism; Male; Receptors; Severity of Illness Index; Transcriptome; Up-Regulation; Wound Healing - genetics; Wound Healing - immunology; Keratinocytes; Receptors; Inflammation; Cytokines; Burns; Gene expression
• ISSN: 0305-4179; 1879-1409
• DOI: 10.1016/j.burns.2013.11.022

Abstract Introduction At all stages of wound healing, growth factors and cytokines play a particularly important role in the interaction with keratinocytes cellular receptors. Keratinocytes have received little attention about their potential to act as a source and target of cytokines. Changes in the cytokine levels after the burning occur prior to the metabolic abnormalities. Thus, it may be possible to develop therapeutic interventions that can mitigate the acute inflammatory response and modulating expression of these cytokines. The objective was to evaluate the expression of 84 genes mediators of the inflammatory response by using PCR array in a primary human epidermal cultured keratinocytes from patients with burns. Methods Keratinocytes cultured from normal skin around injury from small and large burn patient were treated for DNA synthesis. The samples were analyzed by the PCR Superarray® assay and curve analyses were performed for 84 relevant human genes and their involvement in the inflammatory cytokines pathway and receptors. These genes were checked for the up or down regulation. And it was used MetaCore™ for the analysis of networks and Gene Ontology (GO) processes. Results Chemokines of the CXC family were more expressed in the large burn group, except CXCL12. The C, CC and CX3 C chemokine family were downregulated, especially in the small burn group. The interleukins IL8 and IL1B were more expressed in large burn than in small burn; except IL13RA1, IL13 and IL5RA that were downregulated, mainly in the small burn group. Conclusions The cytokine profile showed some important differences between the large and small burn patients, and from this original database, we can create new interventional trials in acute inflammation in burns.