Cloning and regiospecificity studies of two flavonoid glucosyltransferases from Allium cepa

• Published in: Phytochemistry (Oxford) Vol. 64; no. 6; pp. 1069 - 1076
• Main Authors: Kramer, Catherine M., Prata, Rogerio T.N., Willits, Michael G., De Luca, Vincenzo, Steffens, John C., Graser, Gerson
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01.11.2003; Elsevier
• Subjects: Alliaceae; Allium cepa; Amino Acid Sequence; amino acid sequences; binding sites; Biological and medical sciences; Cloning, Molecular; complementary DNA; DNA, Complementary - genetics; enzyme activity; Enzymes; Escherichia coli; Flavonoids; Flavonoids - chemistry; Flavonoids - metabolism; Fundamental and applied biological sciences. Psychology; glucosyltransferase; Glucosyltransferases; Glucosyltransferases - chemistry; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; Glycosylation; hexosyltransferases; Metabolism; molecular cloning; Molecular Sequence Data; nucleotide sequences; Onion; onions; Onions - enzymology; Plant physiology and development; plant proteins; Quercetin; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Regiospecificity; sequence analysis; Sequence Homology, Amino Acid; Stereoisomerism; Substrate Specificity; vegetable crops; Allium cepa; Flavonoids; Onion; Substrate specificity; Quercetin; Glucosyltransferases; Alliaceae; Regiospecificity; Monocotyledones; Quecetin; Enzyme; Transferases; Glycosyltransferases; Biosynthesis; Glucose; Flavonoid; Glucosyltransferases: Substrate specificity; Regiospecifieity; Liliaceae; Angiospermae; Spermatophyta; Recombinant protein

Two UDP-glucose-dependent flavonoid glucosyltransferases (EC 2.4.1.-) isolated from the epidermal layer of yellow onion ( Allium cepa) were functionally expressed in Escherichia coli and their substrate specificity investigated. The two enzymes exhibited different substrate- and regio-specificity pr...