Enhancement of catalytic performance of a metagenome-derived thermophilic oligosaccharide-specific xylanase by binding module removal and random mutagenesis

• Published in: Journal of bioscience and bioengineering Vol. 131; no. 1; pp. 13 - 19
• Main Authors: Boonyapakron, Katewadee, Chitnumsub, Penchit, Kanokratana, Pattanop, Champreda, Verawat
• Format: Journal Article
• Language: English
• Published: Japan Elsevier B.V 01.01.2021
• Subjects: Biocatalysis; biocatalysts; biorefining; carbohydrate binding; catalytic activity; Catalytic domain; Cellulose - metabolism; Directed evolution; Endo-1,4-beta Xylanases - genetics; Endo-1,4-beta Xylanases - metabolism; fluorometry; Gene Library; genomic libraries; health services; Hydrolysis; Kinetics; Metagenome; Mutagenesis; Oligosaccharides - metabolism; Saccharum - metabolism; Substrate Specificity; sugarcane bagasse; Temperature; xylan; Xylanase; xylanases; Xylans - metabolism; Xylo-oligosaccharide; Xylo-oligosaccharide; Catalytic domain; Directed evolution; Xylanase; Metagenome

Xylo-oligosaccharide (XO) is a promising pre-biotic with applications in food, feed and healthcare products. XO can be produced by enzymatic digestion of xylan with xylanase. In this study, we aimed to improve the biochemical properties relevant to catalysis and kinetics of X11, a thermophilic glyco...