Kinetic disruption of lipid rafts is a mechanosensor for phospholipase D

• Published in: Nature communications Vol. 7; no. 1; pp. 13873 - 8
• Main Authors: Petersen, E. Nicholas, Chung, Hae-Won, Nayebosadri, Arman, Hansen, Scott B.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.12.2016; Nature Publishing Group; Nature Portfolio
• Subjects: 14/63; 631/45/173; 631/45/287/1192; 631/57/2270; 631/80/86/2365; 96/10; 96/106; Animals; Biological membranes; Cell Line; Cholesterol; Energy; Enzymes; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; HEK293 Cells; Humanities and Social Sciences; Humans; Hypotheses; Kinetic energy; Kinetics; Lipids; Localization; Mechanotransduction, Cellular; Membrane Microdomains - metabolism; Mice; multidisciplinary; Myoblasts - cytology; Myoblasts - metabolism; Phospholipase D - genetics; Phospholipase D - metabolism; Plasma; Proteins; Science; Science (multidisciplinary); Signal Transduction; Substrate Specificity; Time-Lapse Imaging - methods

The sensing of physical force, mechanosensation, underlies two of five human senses—touch and hearing. How transduction of force in a membrane occurs remains unclear. We asked if a biological membrane could employ kinetic energy to transduce a signal absent tension. Here we show that lipid rafts are...