Leptin impairs myogenesis in C2C12 cells through JAK/STAT and MEK signaling pathways

• Published in: Cytokine (Philadelphia, Pa.) Vol. 61; no. 2; pp. 445 - 454
• Main Authors: Pijet, Maja, Pijet, Barbara, Litwiniuk, Anna, Pajak, Beata, Gajkowska, Barbara, Orzechowski, Arkadiusz
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2013
• Subjects: 1-Phosphatidylinositol 3-kinase; Adipose tissue; AKT protein; Animals; C2C12 muscle cells; Cell Differentiation; Cell Differentiation - drug effects; Cell Line; Cell Survival; Cell Survival - drug effects; cell viability; Cytokines; cytology; Cytoprotection; Cytoprotection - drug effects; Differentiation; drug effects; enzymology; Flavonoids; Flavonoids - pharmacology; Glycogen Synthase Kinase 3; Glycogen Synthase Kinase 3 - metabolism; Glycogen Synthase Kinase 3 beta; Insulin; Insulin - pharmacology; Interferon-gamma; Interferon-gamma - pharmacology; Janus Kinases; Janus Kinases - metabolism; Lean body mass; Leptin; Leptin - pharmacology; MAP kinase; MAP Kinase Signaling System; MAP Kinase Signaling System - drug effects; metabolic inhibitors; metabolism; Mice; Mitogen-Activated Protein Kinase Kinases; Mitogen-Activated Protein Kinase Kinases - metabolism; mitogenesis; Mitogens; Mitogens - pharmacology; muscle development; Muscle Development - drug effects; muscle fibers; muscles; Myoblasts; Myoblasts - cytology; Myoblasts - drug effects; Myoblasts - enzymology; MyoD Protein; MyoD Protein - metabolism; Myogenesis; Myogenin; Myogenin - metabolism; Myotubes; Obesity; pharmacology; Phosphatidylinositol 3-Kinases; Phosphatidylinositol 3-Kinases - metabolism; Phosphorylation; Phosphorylation - drug effects; protein synthesis; Proteins; Signal transduction; Skeletal muscle; SOCS3/JAK/STAT3 pathway; Stat3 protein; STAT3 Transcription Factor; STAT3 Transcription Factor - metabolism; STAT5 Transcription Factor; STAT5 Transcription Factor - metabolism; Suppressor of Cytokine Signaling 3 Protein; Suppressor of Cytokine Signaling Proteins; Suppressor of Cytokine Signaling Proteins - metabolism; Sus scrofa; Transcription, Genetic; Transcription, Genetic - drug effects; white adipose tissue; SOCS3/JAK/STAT3 pathway; Insulin; Leptin; Myogenesis; C2C12 muscle cells

Schematic diagram showing the molecular mechanisms of leptin-dependent repression of myogenic differentiation. Leptin interacts with functional Ob-R to activate JAK/STAT and Ras/MEK but PI3-K/AKT signaling pathways. JAK/STAT stimulates proliferation/mitogenicity whereas MEK lowers both viability and...