Characterization of Mannuronan C-5-Epimerase Genes from the Brown Alga Laminaria digitata

• Published in: Plant physiology (Bethesda) Vol. 133; no. 2; pp. 726 - 735
• Main Authors: Nyvall, Pi, Erwan Corre, Claire Boisset, Tristan Barbeyron, Sylvie Rousvoal, Delphine Scornet, Kloareg, Bernard, Catherine Boyen
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.10.2003; American Society of Plant Physiologists
• Subjects: Agronomy. Soil science and plant productions; Algae; Alginates; Amino Acid Sequence; Bacteria; Biological and medical sciences; Carbohydrate Epimerases - chemistry; Carbohydrate Epimerases - classification; Carbohydrate Epimerases - genetics; Cell walls; Classical and quantitative genetics. Population genetics. Molecular genetics; Cloning, Molecular; Complementary DNA; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Generalities. Genetics. Plant material; Genes, Plant; Genes. Genome; Genetics and breeding of economic plants; Genetics, Genomics, and Molecular Evolution; Laminaria - classification; Laminaria - enzymology; Laminaria - genetics; Laminaria digitata; mannuronan C-5-epimerase; Marine; Models, Molecular; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Open reading frames; Phaeophyceae; Phylogeny; Plants; Protein Conformation; Protoplasts; Protoplasts - enzymology; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Sequence Homology, Amino Acid; Sporophytes; Viruses; Algae; Phylogenetic tree; Biological evolution; Marine plant; Biosynthesis; Metabolic pathway; Alginates; Gene expression; Characterization; Heterokontophyta; Gene; Bacteria; Molecular cloning; Laminaria digitata; Comparative study; Phaeophyceae; Thallophyta

Alginate is an industrially important polysaccharide obtained commercially by harvesting brown algae. The final step in alginate biosynthesis, the epimerization of β-1,4-D-mannuronic acid to α-1,4-L-guluronic acid, a structural change that controls the physicochemical properties of the alginate, is...