Nickel (II)-induced cytotoxicity and apoptosis in human proximal tubule cells through a ROS- and mitochondria-mediated pathway

• Published in: Toxicology and applied pharmacology Vol. 259; no. 2; pp. 177 - 186
• Main Authors: Wang, Yi-Fen, Shyu, Huey-Wen, Chang, Yi-Chuang, Tseng, Wei-Chang, Huang, Yeou-Lih, Lin, Kuan-Hua, Chou, Miao-Chen, Liu, Heng-Ling, Chen, Chang-Yu
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.03.2012; Elsevier
• Subjects: 60 APPLIED LIFE SCIENCES; ACETATES; Acetates - antagonists & inhibitors; Acetates - toxicity; acetylcysteine; Acetylcysteine - pharmacology; APOPTOSIS; Apoptosis - drug effects; Apoptosis - physiology; Biological and medical sciences; carcinogenicity; CARCINOGENS; caspases; Caspases - metabolism; Cell Line; Cell Survival - drug effects; Comet Assay; cytochrome c; Cytotoxicity; DNA Damage; DNA DAMAGES; Flow Cytometry; HK-2 cells; Humans; Kidney Tubules, Proximal - drug effects; Kidney Tubules, Proximal - metabolism; kidneys; LUNGS; Medical sciences; membrane potential; Membrane Potential, Mitochondrial - physiology; MITOCHONDRIA; Mitochondria - drug effects; Mitochondria - metabolism; mitochondrial membrane; NICKEL; Nickel (II); Organometallic Compounds - antagonists & inhibitors; Organometallic Compounds - toxicity; protein synthesis; Proto-Oncogene Proteins c-bcl-2 - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; signal transduction; TOXICITY; Toxicology; TUBULES; Cytotoxicity; Reactive oxygen species; Mitochondria; Nickel (II); HK-2 cells; Apoptosis; Human; Proximal; Oxidative stress; Nickel II; In vitro; Kidney; Renal tubule; Urinary system; Cell death

Nickel compounds are known to be toxic and carcinogenic in kidney and lung. In this present study, we investigated the roles of reactive oxygen species (ROS) and mitochondria in nickel (II) acetate-induced cytotoxicity and apoptosis in the HK-2 human renal cell line. The results showed that the cyto...