Development of a highly thermostable immunoassay based on a nanobody-alkaline phosphatase fusion protein for carcinoembryonic antigen detection

• Published in: Analytical and bioanalytical chemistry Vol. 412; no. 8; pp. 1723 - 1728
• Main Authors: Lin, Jingtao, Yu, Jianli, Wang, Huan, Xu, Yanru, Li, Fei, Chen, Xiaoheng, Liang, Yunlong, Tang, Jinsong, Wu, Lili, Zhou, Zhengwei, Chen, Cailing, Liu, Minjuan, Chun, Xuan, Nian, Rui, Song, Haipeng
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2020; Springer; Springer Nature B.V
• Subjects: Affinity; Alkaline phosphatase; Alkaline Phosphatase - metabolism; Analytical Chemistry; antigen detection; Antigens; Biochemistry; calves; Carcinoembryonic antigen; Carcinoembryonic Antigen - immunology; Carcinoembryonic Antigen - metabolism; CEA (Oncology); Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Characterization and Evaluation of Materials; Chemiluminescence; Chemistry; Chemistry and Materials Science; Communication; cross reaction; Cross-reactivity; detection limit; Display devices; Enzymatic activity; enzyme activity; Enzyme-linked immunosorbent assay; Enzymes; Food Science; Fusion protein; GPI-Linked Proteins - immunology; HEK293 Cells; Humans; Immunoassay; Immunoenzyme Techniques - methods; Intestine; intestines; kidneys; Laboratory Medicine; Limit of Detection; Linearity; Luminescence; Monitoring/Environmental Analysis; Monoclonal antibodies; Nanobodies; neoplasms; Organic chemistry; Phages; Phosphatase; Phosphatases; Placenta; Placental alkaline phosphatase; Proteins; Pyrococcus; Recombinant Fusion Proteins - metabolism; Relative humidity; Reproducibility of Results; Single-Domain Antibodies; thermal stability; China; Thermostability; Nanobody; Alkaline phosphatase; Carcinoembryonic antigen; Chemiluminescent enzyme immunoassay

Carcinoembryonic antigen–related cell adhesion molecule 5 (CEACAM-5) assays are employed in routine clinical settings to diagnose tumor. We selected two nanobodies with high-affinity to CEACAM-5, termed Nb11C12 and Nb2D5, using phage-display technology. The Nb2D5 fused with calf intestinal alkaline...