Development of a highly thermostable immunoassay based on a nanobody-alkaline phosphatase fusion protein for carcinoembryonic antigen detection - Lin, Jingtao; Yu, Jianli; Wang, Huan; Xu, Yanru; Li, Fei; Chen, Xiaoheng; Liang, Yunlong; Tang, Jinsong; Wu, Lili; Zhou, Zhengwei; Chen, Cailing; Liu, Minjuan; et al. (Analytical and bioanalytical chemistry, 2020)
Keywords: Affinity; Alkaline phosphatase; Alkaline Phosphatase - metabolism; Analytical Chemistry; antigen detection; Antigens; Biochemistry; calves; Carcinoembryonic antigen; Carcinoembryonic Antigen - immunology; Carcinoembryonic Antigen - metabolism; CEA (Oncology); Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Characterization and Evaluation of Materials; Chemiluminescence; Chemistry; Chemistry and Materials Science; Communication; cross reaction; Cross-reactivity; detection limit; Display devices; Enzymatic activity; enzyme activity; Enzyme-linked immunosorbent assay; Enzymes; Food Science; Fusion protein; GPI-Linked Proteins - immunology; HEK293 Cells; Humans; Immunoassay; Immunoenzyme Techniques - methods; Intestine; intestines; kidneys; Laboratory Medicine; Limit of Detection; Linearity; Luminescence; Monitoring/Environmental Analysis; Monoclonal antibodies; Nanobodies; neoplasms; Organic chemistry; Phages; Phosphatase; Phosphatases; Placenta; Placental alkaline phosphatase; Proteins; Pyrococcus; Recombinant Fusion Proteins - metabolism; Relative humidity; Reproducibility of Results; Single-Domain Antibodies; thermal stability; China; Thermostability; Nanobody; Alkaline phosphatase; Carcinoembryonic antigen; Chemiluminescent enzyme immunoassay