GPKOW is essential for pre-mRNA splicing in vitro and suppresses splicing defect caused by dominant-negative DHX16 mutation in vivo

• Published in: Bioscience reports Vol. 34; no. 6; p. e00163
• Main Authors: Zang, Shengbing, Lin, Ting-Yu, Chen, Xinji, Gencheva, Marieta, Newo, Alain N. S., Yang, Lixin, Rossi, Daniel, Hu, Jianda, Lin, Shwu-Bin, Huang, Aimin, Lin, Ren-Jang
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd The Biochemical Society 01.01.2014; Portland Press Ltd
• Subjects: Antibodies; Arabidopsis; Binding Sites - genetics; Blotting, Western; Defects; Depletion; Electrophoretic Mobility Shift Assay; Glycerol; Glycine; HEK293 Cells; HeLa Cells; Humans; mRNA; Mutants; Mutation; Original Paper; Peptides; Protein Binding; Proteins; Resins; RNA - genetics; RNA - metabolism; RNA Helicases - genetics; RNA Helicases - metabolism; RNA Precursors - genetics; RNA Splicing; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Saccharomyces; Spliceosomes - metabolism; Splicing; Two-Hybrid System Techniques

Human GPKOW [G-patch (glycine-rich) domain and KOW (Kyrpides, Ouzounis and Woese) domain] protein contains a G-patch domain and two KOW domains, and is a homologue of Arabidopsis MOS2 and Saccharomyces Spp2 protein. GPKOW is found in the human spliceosome, but its role in pre-mRNA splicing remains t...