Simultaneous extraction and determination of mono-/polyglutamyl folates using high-performance liquid chromatography-tandem mass spectrometry and its applications in starchy crops

• Published in: Analytical and bioanalytical chemistry Vol. 411; no. 13; pp. 2891 - 2904
• Main Authors: Wan, Xing, Han, Li-Da, Yang, Min, Zhang, Hong-Yang, Zhang, Chun-Yi, Hu, Ping
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2019; Springer; Springer Nature B.V
• Subjects: alpha-amylase; alpha-Amylases - chemistry; Analysis; Analytical Chemistry; Aspergillus oryzae - enzymology; Biochemistry; boiling; Centrifugation; Cereal crops; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Chromatography, High Pressure Liquid - methods; Corn; Crops; detection limit; Evaluation; filtration; Folic acid; Food Science; Germination; High performance liquid chromatography; hydrolysis; Laboratory Medicine; Limit of Detection; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolism; Methods; Monitoring/Environmental Analysis; Polyglutamic Acid - analysis; Potatoes; Properties; proteinases; Quantitation; regression analysis; Research Paper; Scientific imaging; Seeds - chemistry; Seeds - growth & development; Species; Spectroscopy; Streptomyces griseus - enzymology; Sweet potatoes; tandem mass spectrometry; Tandem Mass Spectrometry - methods; Tetrahydrofolates - analysis; vitamin metabolism; Zea mays - chemistry; Zea mays - growth & development; α-Amylase; China; High-performance liquid chromatography-tandem mass spectrometry; Starchy crops; Polyglutamyl 5-methyltetrahydrofolate; Maize germination; Folate quantification; Polyglutamyl 5-formyltetrahydrofolate

Folates are typically present in polyglutamyl form in organisms. In traditional extraction methods, polyglutamyl folates are hydrolyzed to monoglutamates, sacrificing valuable information. To advance folate metabolism research, we developed an accurate, sensitive, and reproducible extraction method...