Optimizing high-resolution melting analysis for the detection of mutations of GPR30/GPER-1 in breast cancer

• Published in: Gene Vol. 501; no. 2; pp. 118 - 126
• Main Authors: Aihara, Masamune, Yamamoto, Shigeru, Nishioka, Hiroko, Inoue, Yutaro, Hamano, Kimikazu, Oka, Masaaki, Mizukami, Yoichi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.06.2012
• Subjects: Adult; Aged; Aged, 80 and over; Base Sequence; Breast cancer; breast neoplasms; Breast Neoplasms - genetics; DNA; DNA Mutational Analysis; DNA Mutational Analysis - methods; DNA primers; Dyes; estrogen receptors; Estrogens; Female; G protein-coupled receptors; genetics; genomics; GPR30; Guanine nucleotide-binding protein; heterozygosity; Heterozygotes; High resolution melting; homozygosity; Homozygotes; Humans; Magnesium; magnesium chloride; Melting; messenger RNA; methods; Middle Aged; Molecular Sequence Data; mRNA; Mutation; Nucleic Acid Denaturation; Nucleotides; Open reading frame; Open Reading Frames; Point mutation; Polymerase chain reaction; Primers; Receptors, Estrogen; Receptors, Estrogen - genetics; Receptors, G-Protein-Coupled; Receptors, G-Protein-Coupled - genetics; Saturation DNA binding dye; Single-nucleotide polymorphism; Taq polymerase; temperature; Temperature effects; Tumor cell lines; ORF; HRM; Platinum Taq; GPR30; GPER-1; Breast cancer; Expand Hi Fi; Saturation DNA binding dye; Taq polymerase; Open reading frame; SNP; High resolution melting; HotStarTaq; Ex Taq

G protein-coupled receptor 30/G protein estrogen receptor-1 (GPR30/GPER-1) is a novel membrane receptor for estrogen whose mRNA is expressed at high levels in estrogen-dependent cells such as breast cancer cell lines. However, mutations in GRP30 related to diseases remain unreported. To detect unkno...