Nitrous oxide emission potentials of Burkholderia species isolated from the leaves of a boreal peat moss Sphagnum fuscum

• Published in: Bioscience, biotechnology, and biochemistry Vol. 79; no. 12; pp. 2086 - 2095
• Main Authors: Nie, Yanxia, Li, Li, Wang, Mengcen, Tahvanainen, Teemu, Hashidoko, Yasuyuki
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.12.2015
• Subjects: Acetylene - pharmacology; biotechnology; Burkholderia; Burkholderia - chemistry; Burkholderia - drug effects; Burkholderia - isolation & purification; Burkholderia - metabolism; denitrification; genes; Hydrogen-Ion Concentration; nitrous oxide; nitrous oxide (N; Nitrous Oxide - chemistry; Nitrous Oxide - metabolism; nitrous oxide production; nosZ gene; O) emitter; oxidoreductases; phyllosphere; Plant Leaves - microbiology; Sphagnopsida - microbiology; Sphagnum fuscum; Sphagnum moss polyphenols; sucrose; Sucrose - pharmacology; Temperature; Sphagnum moss polyphenols; Sphagnum fuscum; nosZ gene; Burkholderia; nitrous oxide (N2O) emitter
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1080/09168451.2015.1061420

Using a culture-based nitrous oxide (N 2 O) emission assay, three active N 2 O emitters were isolated from Sphagnum fuscum leaves and all identified as members of Burkholderia. These isolates showed N 2 O emission in the medium supplemented with but not with , and Burkholderia sp. SF-E2 showed the most efficient N 2 O emission (0.20 μg·vial −1 ·day −1 ) at 1.0 mM KNO 3 . In Burkholderia sp. SF-E2, the optimum pH for N 2 O production was 5.0, close to that of the phyllosphere of Sphagnum mosses, while the optimum temperature was uniquely over 30 °C. The stimulating effect of additional 1.5 mM sucrose on N 2 O emission was ignorable, but Burkholderia sp. SF-E2 upon exposure to 100 mg·L −1 E-caffeic acid showed uniquely 67-fold higher N 2 O emission. All of the three N 2 O emitters were negative in both acetylene inhibition assay and PCR assay for nosZ-detection, suggesting that N 2 O reductase or the gene itself is missing in the N 2 O-emitting Burkholderia. Upon exposure to E-caffeic acid often contained in Sphagnum mosses, N 2 O emission by Burkholderia sp. SF-E2 that was isolated as an epiphyte of Sphagnum fuscum was highly accelerated.