Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing

• Published in: Journal of industrial microbiology & biotechnology Vol. 44; no. 3; pp. 339 - 351
• Main Authors: Kadisch, Marvin, Schmid, Andreas, Bühler, Bruno
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Oxford University Press 01.03.2017; Springer Berlin Heidelberg
• Subjects: alkanes; biocatalysis; Biocatalysis - Original Paper; Biochemistry; Biodiesel fuels; Biofuels; Bioinformatics; Biomedical and Life Sciences; bioprocessing; Bioreactors; Biotechnology; Biotin; Biotransformation; Catalysis; Chemicals; E coli; Enzymes; Escherichia coli; Escherichia coli - enzymology; Escherichia coli K12; Esters; Esters - metabolism; fatty acid methyl esters; Fatty acids; Fatty Acids - metabolism; feedstocks; free fatty acids; Gene expression; Gene Knockout Techniques; Genetic Engineering; Glucose; Hydrolases - genetics; Hydrolases - metabolism; Hydrolysis; Industrial Microbiology; Inorganic Chemistry; Life Sciences; Metabolic Engineering; Metabolism; Microbiology; Microorganisms; Oxidation; plant fats and oils; Plasmids; Polymers; Fatty acid methyl ester hydrolysis; Metabolic engineering; Industrial biotechnology; Whole-cell biocatalysis; BioH
• ISSN: 1367-5435; 1476-5535; 1476-5535
• DOI: 10.1007/s10295-016-1890-z

Abstract Fatty acid methyl esters (FAMEs) originating from plant oils are most interesting renewable feedstocks for biofuels and bio-based materials. FAMEs can also be produced and/or functionalized by engineered microbes to give access to, e.g., polymer building blocks. Yet, they are often subject...