LC–MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 879; no. 11; pp. 707 - 715
• Main Authors: Lattanzio, Veronica M.T., Solfrizzo, Michele, De Girolamo, Annalisa, Chulze, Sofía N., Torres, Adriana M., Visconti, Angelo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2011; Elsevier
• Subjects: Adult; Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological; Biological and medical sciences; chromatography; Chromatography, Liquid - methods; Conjugates; De-epoxydeoxynivalenol; Deoxynivalenol; Excretion; Female; Fundamental and applied biological sciences. Psychology; General pharmacology; Glucuronide; Human; Human urine; Humans; hydrolysis; in vivo studies; isomers; LC-MS-MS; Male; Matrices; Medical sciences; Metabolites; Middle Aged; monitoring; Mycotoxins; Pharmacology. Drug treatments; Rat urine; Rats; Rats, Wistar; Reproducibility of Results; Sensitivity and Specificity; solid phase extraction; Tandem Mass Spectrometry - methods; Trichothecenes - urine; Urine; Rat urine; Deoxynivalenol; LC–MS/MS; Human urine; De-epoxydeoxynivalenol; Glucuronide; Biological fluid; Rat; Mycotoxin; Vomitoxin; HPLC chromatography; Glucuroconjugate; Characterization; Sesquiterpenes; LC―MS/MS; Human; Urine; Terpenoid; Rodentia; Elimination; Trichothecene; Toxin; Vertebrata; Mammalia; Animal; Mass spectrometry MS/MS; Pharmacokinetics

The understanding of mycotoxins transfer to biological fluids is challenged by the difficulties in performing and replicating in vivo experiments as well as the lack of suitable methods of analysis to detect simultaneously a range of chemically different metabolites at trace levels. LC–MS/MS has bee...