Cell Cycle-Dependent Binding Modes of the Ran Exchange Factor RCC1 to Chromatin

• Published in: Biophysical journal Vol. 104; no. 8; pp. 1642 - 1651
• Main Authors: Bierbaum, Martin, Bastiaens, Philippe I.H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.04.2013; Biophysical Society; The Biophysical Society
• Subjects: Cell Biophysics; Cell Cycle; Cell Cycle Proteins - metabolism; Chromatin; Chromatin - metabolism; Correlation analysis; Cytoplasm; Enzymes; Fluorescence; Guanine Nucleotide Exchange Factors - metabolism; HeLa Cells; Humans; Kinetics; Mitosis; Nuclear Proteins - metabolism; Protein Binding; ran GTP-Binding Protein - metabolism

The formation of an activity gradient of the small G-protein Ran around chromatin depends on the differential partitioning of the opposing enzyme activities of the Ran guanine nucleotide exchange factor RCC1 that resides on chromatin, and the cytoplasmic Ran GTPase activating protein RanGAP. We stud...