Efficient and accurate determination of genome-wide DNA methylation patterns in Arabidopsis thaliana with enzymatic methyl sequencing

• Published in: Epigenetics & chromatin Vol. 13; no. 1; pp. 42 - 17
• Main Authors: Feng, Suhua, Zhong, Zhenhui, Wang, Ming, Jacobsen, Steven E.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.10.2020; BioMed Central; BMC
• Subjects: APOBEC; Arabidopsis; Arabidopsis thaliana; Base composition; Bisulfite; Bisulfite sequencing; Cytosine; Deoxyribonucleic acid; DNA; DNA Methylation; DNA sequencing; EM-seq; Enzymes; Epigenetic inheritance; Epigenetics; Epigenome; Flowers; Gene mapping; Genetic research; Genome, Plant; Genomes; Genomics; Methylation; Nucleotide sequence; Polymerase chain reaction; Pyrimidines; Sensitivity and Specificity; Sequence Analysis, DNA - methods; Sequence Analysis, DNA - standards; Sulfites; TET; WGBS; TET; Arabidopsis flowers; Bisulfite sequencing; APOBEC; DNA methylation; Arabidopsis leaves; WGBS; EM-seq

5' methylation of cytosines in DNA molecules is an important epigenetic mark in eukaryotes. Bisulfite sequencing is the gold standard of DNA methylation detection, and whole-genome bisulfite sequencing (WGBS) has been widely used to detect methylation at single-nucleotide resolution on a genome...