PKC-mediated secretion of death factors in LNCaP prostate cancer cells is regulated by androgens

• Published in: Molecular carcinogenesis Vol. 48; no. 3; pp. 187 - 195
• Main Authors: Xiao, Liqing, Gonzalez-Guerrico, Anatilde, Kazanietz, Marcelo G.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.03.2009
• Subjects: androgens; apoptosis; Apoptosis - drug effects; Blotting, Western; Cell Line, Tumor; Culture Media, Conditioned - pharmacology; Enzyme-Linked Immunosorbent Assay; Humans; JNK; JNK Mitogen-Activated Protein Kinases - metabolism; Male; Metribolone - pharmacology; p38 MAPK; p38 Mitogen-Activated Protein Kinases - metabolism; PKC; PMA; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Protein Kinase C-delta - antagonists & inhibitors; Protein Kinase C-delta - genetics; Protein Kinase C-delta - metabolism; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; RNA, Neoplasm - genetics; RNA, Neoplasm - metabolism; RNA, Small Interfering - pharmacology; Signal Transduction; Tetradecanoylphorbol Acetate - pharmacology; TNF-Related Apoptosis-Inducing Ligand - genetics; TNF-Related Apoptosis-Inducing Ligand - metabolism; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism

Activation of PKCδ in androgen‐dependent LNCaP prostate cancer cells leads to apoptosis via the activation of p38 MAPK and JNK cascades. We have recently shown that treatment of LNCaP cells with phorbol 12‐myristate 13‐acetate (PMA) leads to a PKCδ‐mediated autocrine release of death factors, includ...