Draft genome sequence of the oomycete Pythium destruens strain ATCC 64221 from a horse with pythiosis in Australia

• Published in: BMC research notes Vol. 13; no. 1; p. 329
• Main Authors: Krajaejun, Theerapong, Kittichotirat, Weerayuth, Patumcharoenpol, Preecha, Rujirawat, Thidarat, Lohnoo, Tassanee, Yingyong, Wanta
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 09.07.2020; BioMed Central; BMC
• Subjects: Animals; Australia; Bioinformatics; Biology; Comparative analysis; Data Note; Deoxyribonucleic acid; Diagnosis; DNA; DNA sequencing; Genes; Genetic aspects; Genome; Genome sequence; Genomes; Genomics; Health aspects; High-Throughput Nucleotide Sequencing; Horse Diseases; Horses; Infectious diseases; Methods; Next-generation sequencing; Nucleotide sequence; Pathogenesis; Pathogens; Pythiosis; Pythium; Pythium - genetics; Pythium - isolation & purification; Pythium destruens; Pythium insidiosum; Sequence Analysis, DNA; Vaccines; Varieties; Water molds; Australia; Brazil; Thailand; United States > US; Pythium insidiosum; Australia; Genome sequence; Pythiosis; Pythium destruens
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-020-05168-1

Genome sequences are a vital resource for accelerating the biological exploration of an organism of interest. Pythium destruens (a synonym of Pythium insidiosum) causes a difficult-to-treat infectious disease called pythiosis worldwide. Detection and management of pythiosis are challenging. Basic knowledge of the disease is lacking. Genomes of this organism isolated from different continents (i.e., Asia and the Americas) have been sequenced and publicly available. Here, we sequenced the genome of an Australian isolate of P. destruens. Genome data will facilitate the comparative analysis of this and related species at the molecular level. Genomic DNA of the P. destruens strain ATCC 64221, isolated from a horse with pythiosis in Australia, was used to prepare one paired-end library (with 180-bp insert) for next-generation sequencing, using the Illumina HiSeq 2500 short-read platform. Raw reads were cleaned and assembled by several bioinformatics tools. A total of 20,860,454 processed reads, accounted for 2,614,890,553 total bases, can be assembled into a 37.8-Mb genome, consisting 13,060 contigs (average length: 2896 bases; range: 300-142,967), N of 11,370 bases, and 2.9% 'N' composition. The genome was determined 85.9% completeness, contained 14,424 predicted genes, and can be retrieved online at the NCBI/DDBJ databases under the accession number BCFQ01000000.1.