Stem-Loop RT-qPCR as an Efficient Tool for the Detection and Quantification of Small RNAs in Giardia lamblia

• Published in: Genes Vol. 7; no. 12; p. 131
• Main Authors: Marcial-Quino, Jaime, Gómez-Manzo, Saúl, Fierro, Francisco, Vanoye-Carlo, America, Rufino-González, Yadira, Sierra-Palacios, Edgar, Castillo-Villanueva, Adriana, Castillo-Rodríguez, Rosa, Rodríguez-Bustamante, Eduardo, Arreguin-Espinosa, Roberto, Reyes-Vivas, Horacio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.12.2016; MDPI
• Subjects: Giardia lamblia; microRNA; Giardia lamblia; stem-loop RT-qPCR; small nucleolar RNA

Stem-loop quantitative reverse transcription PCR (RT-qPCR) is a molecular technique used for identification and quantification of individual small RNAs in cells. In this work, we used a Universal ProbeLibrary (UPL)-based design to detect—in a rapid, sensitive, specific, and reproducible way—the smal...