Proteomic analysis upon peach fruit infection with Monilinia fructicola and M. laxa identify responses contributing to brown rot resistance

• Published in: Scientific reports Vol. 10; no. 1; p. 7807
• Main Authors: Papavasileiou, Antonios, Tanou, Georgia, Samaras, Anastasios, Samiotaki, Martina, Molassiotis, Athanassios, Karaoglanidis, George
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.05.2020; Nature Publishing Group
• Subjects: 1-Aminocyclopropane-1-carboxylate deaminase; 631/1647; 631/326; 631/449; Allergens; Ascomycota - pathogenicity; Brown rot; Cultivars; Disease resistance; Disease Resistance - genetics; Energy metabolism; Formate dehydrogenase; Fruit - genetics; Fruit - growth & development; Fruit - microbiology; Fruits; Gene expression; Glutathione transferase; Hsp70 protein; Humanities and Social Sciences; Hydrolase; Infections; Inoculation; Latex; Metabolism; Monilinia; Monilinia fructicola; Monilinia laxa; multidisciplinary; Pathogens; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - genetics; Polymerase chain reaction; Proteins; Proteomes; Proteomics - methods; Prunus persica - genetics; Prunus persica - growth & development; Prunus persica - microbiology; S-Formylglutathione hydrolase; Science; Science (multidisciplinary); Thaumatin
• DOI: 10.1038/s41598-020-64864-x
• ISSN: 2045-2322

Brown rot, caused by Monilinia spp., is a major peach disease worldwide. In this study, the response of peach cultivars Royal Glory (RG) and Rich Lady (RL) to infection by Monilinia fructicola or Monilinia laxa , was characterized. Phenotypic data, after artificial inoculations, revealed that ‘RL’ was relatively susceptible whereas ‘RG’ was moderately resistant to Monilinia spp. Comparative proteomic analysis identified mesocarp proteins of the 2 cultivars whose accumulation were altered by the 2 Monilinia species. Functional analysis indicated that pathogen-affected proteins in ‘RG’ were mainly involved in energy and metabolism, while, differentially accumulated proteins by the pathogen presence in ‘RL’ were involved in disease/defense and metabolism. A higher number of proteins was differentiated in ‘RG’ fruit compared to ‘RL’. Upon Monilinia spp. infection, various proteins were-down accumulated in ‘RL’ fruit. Protein identification by mass spectrometric analysis revealed that several defense-related proteins including thaumatin, formate dehydrogenase, S -formylglutathione hydrolase, CBS domain-containing protein, HSP70, and glutathione S -transferase were up-accumulated in ‘RG’ fruit following inoculation. The expression profile of selected defense-related genes, such as major latex allergen, 1-aminocyclopropane-1-carboxylate deaminase and UDP-glycoltransferase was assessed by RT-PCR. This is the first study deciphering differential regulations of peach fruit proteome upon Monilinia infection elucidating resistance responses.