RIAM: A Universal Accessible Protocol for the Isolation of High Purity DNA from Various Soils and Other Humic Substances

• Published in: Methods and protocols Vol. 5; no. 6; p. 99
• Main Authors: Pinaev, Alexander G, Kichko, Arina A, Aksenova, Tatiana S, Safronova, Vera I, Kozhenkova, Elena V, Andronov, Evgeny E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.12.2022; MDPI
• Subjects: Acids; Agricultural chemicals; Alcohol; Biotechnology industry; Clay; CTAB DNA precipitation; Deoxyribonucleic acid; DNA; Ethylenediaminetetraacetic acid; Genetic testing; high phosphate; Humic acid; Minerals; Molecular weight; Phosphates; Polyethylene glycol; Polymerase chain reaction; Protocol; soil DNA isolation; Soils; Sorption; United States; United States > US; soil DNA isolation; high phosphate; CTAB DNA precipitation

A single universal open protocol RIAM (named after Research Institute for Agricultural Microbiology) for the isolation of high purity DNA from different types of soils and other substrates (high and low in humic, clay content, organic fertilizer, etc.) is proposed. The main features of the RIAM prot...