GEA 3162, a peroxynitrite donor, induces Bcl-2-sensitive, p53-independent apoptosis in murine bone marrow cells

• Published in: Biochemical pharmacology Vol. 74; no. 7; pp. 1039 - 1049
• Main Authors: Taylor, Emma L., Li, John T., Tupper, Joan C., Rossi, Adriano G., Winn, Robert K., Harlan, John M.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.10.2007; Elsevier Science
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Bcl-2; Biological and medical sciences; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Caspase; Caspases - metabolism; Cell Line; Cell Survival; Dose-Response Relationship, Drug; Enzyme Activation; Medical sciences; Mice; Mitochondria - drug effects; Mitochondria - metabolism; Mitogen-Activated Protein Kinases - metabolism; Myeloid; Nitric Oxide Donors - pharmacology; p53; Peroxynitrite; Pharmacology. Drug treatments; Proto-Oncogene Proteins c-bcl-2 - metabolism; Triazoles - pharmacology; Tumor Suppressor Protein p53 - metabolism; Bcl-2; NO; Peroxynitrite; JNK; Jaws II-GFP; Caspase; MAP kinase; Myeloid; p53; GEA 3162; GFP; Jaws II-Bcl-2; STSP; ONOO; Apoptosis; Enzyme; Cysteine endopeptidases; p53 Protein; Rodentia; In vitro; Peptidases; Vertebrata; Mammalia; TP53 Gene; Donor; Mouse; Cell death; Animal; C-Onc gene; Bone marrow; Hydrolases; Protooncogene; Tumor suppressor gene

Apoptosis may be regulated by oxidants such as peroxynitrite (ONOO−). The tumour suppressor, p53, has been reported to play a crucial role in apoptosis induced by oxidants, therefore we assessed the ability of a ONOO− donor, GEA 3162, to activate caspases and induce mitochondrial permeability in a p...