Detection of promoter methylation of tumor suppressor genes in serum DNA of breast cancer cases and benign breast disease controls

• Published in: Epigenetics Vol. 7; no. 11; pp. 1258 - 1267
• Main Authors: Sturgeon, Susan R., Balasubramanian, Raji, Schairer, Catherine, Muss, Hyman B., Ziegler, Regina G., Arcaro, Kathleen F.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.11.2012; Landes Bioscience
• Subjects: Adult; Aged; Aged, 80 and over; Binding; Biology; Biomarkers, Tumor - blood; Bioscience; breast cancer; Breast Diseases - blood; Breast Diseases - genetics; Breast Neoplasms - blood; Breast Neoplasms - diagnosis; Breast Neoplasms - genetics; Calcium; Cancer; Case-Control Studies; Cell; Cycle; DNA Methylation; DNA, Neoplasm - blood; early marker; Female; Genes, Tumor Suppressor; Humans; Landes; Middle Aged; Organogenesis; promoter methylation; Promoter Regions, Genetic; Proteins; pyrosequencing; Research Paper; serum DNA

Tumors are capable of shedding DNA into the blood stream. This shed DNA may be recovered from serum or plasma. The objective of this study was to evaluate whether pyrosequencing promoter DNA in a panel of 12 breast cancer-related genes (APC, BRCA1, CCND2, CDH1, ESR1, GSTP1, HIN1, P16, RARβ, RASSF1,...