High-level phenol bioproduction by engineered Pichia pastoris in glycerol fed-batch fermentation using an efficient pertraction system

• Published in: Bioresource technology Vol. 393; p. 130144
• Main Authors: Kumokita, Ryota, Bamba, Takahiro, Yasueda, Hisashi, Tsukida, Ayato, Nakagawa, Keizo, Kitagawa, Tooru, Yoshioka, Tomohisa, Matsuyama, Hideto, Yamamoto, Yasuhito, Maruyama, Satoshi, Hayashi, Takahiro, Kondo, Akihiko, Hasunuma, Tomohisa
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2024
• Subjects: batch fermentation; culture media; Glycerol; Hollow fiber membrane contactor; Komagataella pastoris; Pertraction; Phenol; Pichia pastoris; solvents; toxicity; tributyrin; tyrosine; yeasts; Glycerol; Pertraction; Phenol; Pichia pastoris; Hollow fiber membrane contactor

[Display omitted] •Metabolic engineering in Pichia pastoris boosts phenol production.•Created a hollow fiber membrane contactor effective for extracting phenol.•Extracted phenol produced by P. pastoris from the fermentation medium.•Demonstrated the potential of pertraction in producing chemicals tox...