Environmental DNA (eDNA) metabarcoding reveals strong discrimination among diverse marine habitats connected by water movement

• Published in: Molecular ecology resources Vol. 19; no. 2; pp. 426 - 438
• Main Authors: Jeunen, Gert‐Jan, Knapp, Michael, Spencer, Hamish G., Lamare, Miles D., Taylor, Helen R., Stat, Michael, Bunce, Michael, Gemmell, Neil J.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.03.2019
• Subjects: Aquatic habitats; Aquatic Organisms - classification; Aquatic Organisms - genetics; Aquatic Organisms - growth & development; Biodiversity; biodiversity assessment; Biota; Cluster Analysis; Crustacea; Crustaceans; cytochrome-c oxidase; Cytochromes; data collection; Deoxyribonucleic acid; Dispersal; Dispersion; DNA; DNA barcoding; DNA Barcoding, Taxonomic; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Ecosystem; Electron Transport Complex IV - genetics; Environmental DNA; Eukaryota - classification; Eukaryota - genetics; Eukaryota - growth & development; fish; Genera; genes; Habitat preferences; Habitats; marine eDNA; Marine environment; metabarcoding; Metagenomics; Monitoring; Monitoring methods; Ordination; Phylogeny; Polls & surveys; ribosomal RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Shellfish; spatial resolution; surveys; Taxa; Water flow; Water Movements; marine eDNA; spatial resolution; metabarcoding; biodiversity assessment

While in recent years environmental DNA (eDNA) metabarcoding surveys have shown great promise as an alternative monitoring method, the integration into existing marine monitoring programs may be confounded by the dispersal of the eDNA signal. Currents and tidal influences could transport eDNA over g...