Development of a versatile in vitro method for understanding the migration of Fasciola hepatica newly excysted juveniles
Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However,...
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| Published in | Parasitology Vol. 143; no. 1; pp. 24 - 33 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Cambridge, UK
Cambridge University Press
01.01.2016
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However, relatively few studies have concentrated on the mechanisms of invasion of the gut by newly excysted juvenile liver flukes (NEJ) and the host response triggered by this event. The aim of this work was to develop an in vitro model to study invasion by NEJ, while also reducing the requirement for challenge infections of experimental animals. Fasciola hepatica metacercariae were excysted in vitro and placed into compartments containing rat distal jejunal sheets. Variations in incubation medium, chamber size and incubation temperature were used to identify optimal conditions for NEJ migration across the gut. Histological examination showed increased migration until 120 min post-incubation. The use of RPMI, without gassing at 39 °C, as the incubation medium was found to be optimal, with 40·5% of NEJ migrating after 150 min. This study describes a readily-reproducible method for studying the migration of F. hepatica NEJ within the definitive host. It will be useful for identifying potential drug and vaccine targets. |
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| AbstractList | Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However, relatively few studies have concentrated on the mechanisms of invasion of the gut by newly excysted juvenile liver flukes (NEJ) and the host response triggered by this event. The aim of this work was to develop an in vitro model to study invasion by NEJ, while also reducing the requirement for challenge infections of experimental animals. Fasciola hepatica metacercariae were excysted in vitro and placed into compartments containing rat distal jejunal sheets. Variations in incubation medium, chamber size and incubation temperature were used to identify optimal conditions for NEJ migration across the gut. Histological examination showed increased migration until 120 min post-incubation. The use of RPMI, without gassing at 39 °C, as the incubation medium was found to be optimal, with 40·5% of NEJ migrating after 150 min. This study describes a readily-reproducible method for studying the migration of F. hepatica NEJ within the definitive host. It will be useful for identifying potential drug and vaccine targets. SUMMARY Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However, relatively few studies have concentrated on the mechanisms of invasion of the gut by newly excysted juvenile liver flukes (NEJ) and the host response triggered by this event. The aim of this work was to develop an in vitro model to study invasion by NEJ, while also reducing the requirement for challenge infections of experimental animals. Fasciola hepatica metacercariae were excysted in vitro and placed into compartments containing rat distal jejunal sheets. Variations in incubation medium, chamber size and incubation temperature were used to identify optimal conditions for NEJ migration across the gut. Histological examination showed increased migration until 120 min post-incubation. The use of RPMI, without gassing at 39 °C, as the incubation medium was found to be optimal, with 40·5% of NEJ migrating after 150 min. This study describes a readily-reproducible method for studying the migration of F. hepatica NEJ within the definitive host. It will be useful for identifying potential drug and vaccine targets. SUMMARY Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However, relatively few studies have concentrated on the mechanisms of invasion of the gut by newly excysted juvenile liver flukes (NEJ) and the host response triggered by this event. The aim of this work was to develop an in vitro model to study invasion by NEJ, while also reducing the requirement for challenge infections of experimental animals. Fasciola hepatica metacercariae were excysted in vitro and placed into compartments containing rat distal jejunal sheets. Variations in incubation medium, chamber size and incubation temperature were used to identify optimal conditions for NEJ migration across the gut. Histological examination showed increased migration until 120 min post-incubation. The use of RPMI, without gassing at 39 °C, as the incubation medium was found to be optimal, with 40·5% of NEJ migrating after 150 min. This study describes a readily-reproducible method for studying the migration of F. hepatica NEJ within the definitive host. It will be useful for identifying potential drug and vaccine targets. |
| Author | MULCAHY, GRACE GARCIA-CAMPOS, ANDRES BAIRD, ALAN W. |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26521819$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_3390_microorganisms11071708 crossref_primary_10_1017_S003118201700124X crossref_primary_10_3389_fimmu_2019_02081 crossref_primary_10_1016_j_vetpar_2020_109028 crossref_primary_10_1371_journal_ppat_1005991 crossref_primary_10_1016_j_ijpara_2017_02_004 crossref_primary_10_1016_j_pt_2020_09_007 crossref_primary_10_1371_journal_pntd_0010811 crossref_primary_10_1016_j_rvsc_2020_10_011 crossref_primary_10_1016_j_actbio_2021_12_015 crossref_primary_10_1016_j_ijpara_2020_11_005 crossref_primary_10_1016_j_vetpar_2023_109981 |
| Cites_doi | 10.1074/mcp.M900045-MCP200 10.1016/0166-6851(93)90172-T 10.1007/BF02121277 10.1046/j.1365-3024.1999.00226.x 10.1292/jvms.54.69 10.1016/S0022-1759(98)00026-X 10.1016/0014-4894(81)90036-9 10.1016/0014-4894(75)90036-3 10.1080/10611860410001693715 10.1016/j.ijpara.2007.10.007 10.1016/j.biochi.2008.04.020 10.1258/002367787781268693 10.2307/3284586 10.1186/1471-2164-11-227 10.1016/S0034-5288(18)32558-X 10.1016/j.jconrel.2006.06.021 10.1016/S0034-5288(18)33562-8 10.2307/3281496 10.1016/0169-4758(93)90180-N |
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| Copyright | Copyright © Cambridge University Press 2015 Copyright © Cambridge University Press 2015 This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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| Keywords | Fasciola hepatica NEJ jejunum horizontal diffusion chamber gut method optimization |
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| Snippet | Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for... SUMMARY Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of... SUMMARY Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of... |
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| SubjectTerms | Animals Cattle Cattle Diseases - parasitology Disease Models, Animal Fasciola hepatica - physiology Fascioliasis - parasitology Fascioliasis - veterinary Male Rats Rats, Wistar Sheep Sheep Diseases - parasitology |
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| Title | Development of a versatile in vitro method for understanding the migration of Fasciola hepatica newly excysted juveniles |
| URI | https://www.cambridge.org/core/product/identifier/S0031182015001481/type/journal_article https://www.ncbi.nlm.nih.gov/pubmed/26521819 https://www.proquest.com/docview/1749566855/abstract/ https://search.proquest.com/docview/1750429945 |
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