A User’s Guide to Cell-Free Protein Synthesis

• Published in: Methods and protocols Vol. 2; no. 1; p. 24
• Main Authors: Gregorio, Nicole E., Levine, Max Z., Oza, Javin P.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 12.03.2019; MDPI AG
• Subjects: cell-free metabolic engineering (CFME); cell-free protein expression (CFPE); cell-free protein synthesis (CFPS); cell-free synthetic biology; in vitro protein synthesis; in vitro transcription-translation (TX-TL); Review; in vitro protein synthesis; in vitro transcription-translation (TX-TL); cell-free protein synthesis (CFPS); cell-free synthetic biology; cell-free metabolic engineering (CFME); cell-free protein expression (CFPE)

Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on livi...